Fitness costs of resistance to Bacillus thuringiensis in the Indianmeal moth, Plodia interpunctella
نویسندگان
چکیده
منابع مشابه
survay on the resistance of different stages of mediterranean flour moth ( plodia interpunctella) to bacillus thuringiensis
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متن کاملThioredoxin from the Indianmeal Moth Plodia interpunctella: Cloning and Test of the Allergenic Potential in Mice
BACKGROUND/OBJECTIVE The Indianmeal moth Plodia interpunctella is a highly prevalent food pest in human dwellings, and has been shown to contain a number of allergens. So far, only one of these, the arginine kinase (Plo i 1) has been identified. OBJECTIVE The aim of this study was to identify further allergens and characterise these in comparison to Plo i 1. METHOD A cDNA library from whole...
متن کاملDifferent mechanisms of resistance to Bacillus thuringiensis toxins in the indianmeal moth.
Susceptibility to protoxin and toxin forms of Cry1Ab and the binding of (125)I-labeled Cry1Ab and Cry1Ac has been examined in three Plodia interpunctella colonies, one susceptible (688(s)) and two resistant (198(r) and Dpl(r)) to Bacillus thuringiensis. Toxicological studies showed that the 198(r) colony was 11-fold more resistant to Cry1Ab protoxin than to Cry1Ab activated toxin, whereas the D...
متن کاملTrypsinogen-like cDNAs and quantitative analysis of mRNA levels from the Indianmeal moth, Plodia interpunctella.
Two cDNA fragments encoding full-length trypsinogen-like proteins were cloned from larvae of two strains (RC688s and HD198r) of the Indianmeal moth, Plodia interpunctella (Hübner), which differed in their sensitivity to Bacillus thuringiensis protoxins. One cDNA fragment contained 874 nucleotides, including a 780-nucleotide open reading frame that encoded a trypsinogen-like protein (PiT2b). Ano...
متن کاملInsect resistance to Bacillus thuringiensis: alterations in the indianmeal moth larval gut proteome.
Insect resistance to the Cry toxins of Bacillus thuringiensis (Bt) has been examined previously using a number of traditional biochemical and molecular techniques. In this study, we utilized a proteomic approach involving two-dimensional differential gel electrophoresis, mass spectrometry, and function-based activity profiling to examine changes in the gut proteins from the larvae of an Indianm...
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ژورنال
عنوان ژورنال: Entomologia Experimentalis et Applicata
سال: 2000
ISSN: 0013-8703,1570-7458
DOI: 10.1046/j.1570-7458.2000.00707.x